Journal: Allergology international : official journal of the Japanese Society of Allergology

Article Title: Mucus plugging in allergic bronchopulmonary aspergillosis: Implication of the eosinophil DNA traps.

doi: 10.1016/j.alit.2017.08.002

Figure Lengend Snippet: Fig. 1. (A) Papanicolaou staining (scale bar: 10 mm) and (B) DNA and histone H1 co-localization in bronchial secretions before treatment (scale bar: 50 mm). Chromatolytic cells aggregated to form basophilic filaments (A, arrows, see also Supplementary Video 1). (C) Chest CT images (upper panels) and bronchial lavage fluid (BALF) stained with cell- impermeable DNA dye SYTOX Green (lower panels) before and after treatment (scale bars: 50 mm). Inserts in the lower panels indicate higher magnification (scale bars: 20 mm).

Article Snippet: Additionally, bronchial lavage fluid (BALF) from right middle lobe bronchus were stained with cell-impermeable DNA-specific dye SYTOX Green (Invitrogen).

Techniques: Staining

Journal: The journal of physical chemistry. B

Article Title: Amino Acid Stabilization of Nucleic Acid Secondary Structure: Kinetic Insights from Single-Molecule Studies

doi: 10.1021/acs.jpcb.8b06872

Figure Lengend Snippet: DNA hairpin construct used in folding studies.

Article Snippet: The complete DNA oligonucleotide (5′-Cy5-CTTCAGT-A 40 -Cy3-ACTGAAG-A 11 -biotin-3′) is purchased from Integrated DNA Technologies. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 DNA hairpin construct used in folding studies.

Techniques: Construct

Journal: The journal of physical chemistry. B

Article Title: Amino Acid Stabilization of Nucleic Acid Secondary Structure: Kinetic Insights from Single-Molecule Studies

doi: 10.1021/acs.jpcb.8b06872

Figure Lengend Snippet: (a) Single-molecule time traces for hairpin folding with and without 100 mM arginine HCl, exhibiting two-state behavior. Green and red lines correspond to donor (Cy3) and acceptor (Cy5) fluorescence, respectively. Residence durations are used to construct survival time distributions for (b) folding and (c) unfolding. Exponential fits are used to extract rate constants.

Article Snippet: The complete DNA oligonucleotide (5′-Cy5-CTTCAGT-A 40 -Cy3-ACTGAAG-A 11 -biotin-3′) is purchased from Integrated DNA Technologies. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 DNA hairpin construct used in folding studies.

Techniques: Fluorescence, Construct